Alexion Pharmaceuticals, Inc.
METHODS OF PRODUCING ANTI-C5 ANTIBODIES

Abstract:

The present application relates to a method of producing an anti-C5 antibody (ravulizumab). wherein the method comprises: --culturing mammalian cells comprising a nucleic acid encoding the anti-C5 antibody in a cell culture production medium--Performing two or more steps selected from the group consisting of: a recovery step; purification by Protein A affinity chromatography, a low pH viral inactivation step; Purification by cation exchange chromatography; Purification by anion exchange chromatography; a virus reduction filtration step; and a concentration and diafiltration step