Regeneron Pharmaceuticals, Inc.
CHARACTERIZATION OF ADENO-ASSOCIATED VIRUS USING MICROCHIP CAPILLARY ELECTROPHORESIS

Abstract:

Methods and systems for identifying capsid viral proteins in a sample containing viral vectors are provided, including determining the ratio of the capsid viral proteins of adeno-associated virus. The methods and systems comprise denaturing the capsid viral proteins in the sample, labeling the denatured capsid viral proteins with a lysine-conjugation dye, generating a separation profile of the denatured/labeled capsid viral proteins using microchip capillary electrophoresis, quantifying levels of the capsid viral proteins based on the separation profile, determining a quantification ratio of the capsid viral proteins based on the separation profile, and normalizing the quantification ratio based on lysine contents of the capsid viral proteins.