Regeneron Pharmaceuticals, Inc.
Non-human animals having an engineered immunoglobulin .LAMBDA. light chain locus

Abstract:

Non-human animals (and/or non-human cells) and methods of using and making the same are provided, which non-human animals (and/or non-human cells) have a genome comprising human antibody-encoding sequences (i.e., immunoglobulin genes). Non-human animals described herein express antibodies that contain human Ig.lamda. light chains, in whole or in part. In particular, non-human animals provided herein are, in some embodiments, characterized by expression of antibodies that contain human Ig.lamda. light chains, in whole or in part, that are encoded by human Ig.lamda. light chain-encoding sequences inserted into an endogenous Ig.lamda. light chain locus of said non-human animals. Methods for producing antibodies from non-human animals are also provided.