Agilent Technologies, Inc.
Use of Mild Electrophiles to Reduce Artifacts in Analyzing Glycans Released from Glycoproteins or Glycopeptides

Abstract:

The presence of mild electrophiles, such as aldehydes, during the denaturation of glycoproteins or glycopeptides and subsequent enzymatic deglycosylation reduces artifacts in subsequent analyses of the glycans released from the glycoproteins or gly-copeptides.