Agilent Technologies, Inc.
Synthesis of pools of probes by primer extension

Abstract:

Provided herein is a method for making a pool of probes by primer extension. In certain embodiments, the method comprises hybridizing a first population of oligonucleotides comprising a top strand sequence having the following formula V1-B-3' with a second population of oligonucleotides comprising a bottom strand sequence having the following formula V2'-B'-3' to provide a population of duplexes. After hybridizing, the 3' ends of the oligonucleotides in the duplexes are extended to produce a population of double stranded products comprising a top strand sequence having the following formula V1-B-V2, where V2 is complementary to V2'.