Agilent Technologies, Inc.
SEQUENCING AN INSERT AND AN IDENTIFIER WITHOUT DENATURATION

Abstract:

Methods and kits for next-generation sequencing are disclosed. In some embodiments, the present methods comprise sequencing an insert and sequencing an insert without an intervening denaturation step. Decreased sequencing signal from insert sequences, the use of unlabeled nucleotides to form double stranded insert constructs, and the use of synthesis blocking nucleotides are also discussed.