Agilent Technologies, Inc.
METHODS AND KITS TO IMPROVE THE FLUORESCENT SIGNAL OF DMB-LABELED SIALIC ACIDS

Abstract:

The present invention provides methods and kits to improve the signal from sialic acids labeled with the dye 1, 2-diamino-4, 5-methylenedioxybenzene ("DMB"). The methods include labeling the sialic acids with DMB in an aqueous solution comprising the amino acid glycine, with pH adjusted to 1.5-3.2 by an acid, such as phosphoric acid or hydrochloric acid, and a reductant.