Akoya Biosciences, Inc.
MULTIPLEXED IMAGING REAGENT COMPOSITIONS AND KITS

Abstract:

Methods for detecting multiple target analytes in a biological sample include: (a) contacting the biological sample with a plurality of different types of probes, where each different type of probe includes a capture moiety that selectively binds to a different target analyte in the sample, and an oligonucleotide having a sequence that is unique among other types of probes in the plurality of different types of probes; (b) binding an optical label to one of the different types of probes; (c) contacting the sample with a composition that includes at least one blocking agent, where the at least one blocking agent includes an oligonucleotide having a sequence that hybridizes to the oligonucleotide of another type of probe from among the different types of probes; and (d) obtaining an image of the sample that includes information corresponding to one or more locations of the one type of probe in the sample.