Applied Materials, Inc.
POSITIVE PRESSURE DRIVEN FLOW FOR MULTIPLEXED FLUORESCENCE IN SITU HYBRIDIZATION IMAGING SYSTEM

Abstract:

A fluorescent in-situ hybridization imaging system, including a flow cell to contain a sample to be exposed to fluorescent probes in a reagent; a plurality of reagent reservoirs, each reagent reservoir including a container to hold a liquid reagent; a valve system to control flow from one of a plurality of reagent reservoirs to the flow cell; a pressure source coupled to each of the plurality of reagent reservoirs to apply a positive pressure to liquid reagent in the container and urge the liquid reagent to flow toward the flow cell; and a fluorescence microscope including a variable frequency excitation light source and a camera positioned to receive fluorescently emitted light from the sample.