Amgen Inc.
PURIFICATION METHODS FOR GUANINE-RICH OLIGONUCLEOTIDES

Abstract:

The present invention relates to methods for purifying nucleic acids. In particular, the present invention relates to a method for separating guanine-rich oligonucleotides from quadruplex secondary structures formed from the oligonucleotides using monolithic anion exchange chromatography. Mobile phase parameters that control quadruplex formation and enable separation of the intact quadruplex from the single-strand oligonucleotide are also described.