Amgen Inc.
SYSTEMS AND METHODS FOR DETERMINING PROTEIN CONCENTRATIONS OF UNKNOWN PROTEIN SAMPLES BASED ON AUTOMATED MULTI-WAVELENGTH CALIBRATION

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Abstract:

ultraviolet (UV) based imaging method for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration. In various embodiments, a processor receives each of a standard set of wavelength data and an unknown set of wavelength data as recorded by a detector. Each standard set of wavelength data and unknown set of wavelength data defines a series of absorbance-to-wavelength value pairs across a first range of wavelengths selected from a range of a single-wavelength light beams of a UV spectra. The processor generates a multi-wavelength calibration model based on each of a first series of first absorbance-to-wavelength value pairs of the standard set of wavelength data. The processor implements the multi-wavelength calibration model to determine, for each unknown protein sample of the given unknown protein samples, a plurality of protein concentration values.

Status:
Application
Type:

Utility

Filling date:

6 Aug 2020

Issue date:

1 Sep 2022