Becton, Dickinson and Company
MULTIPLEXED SINGLE CELL IMMUNOASSAY

Abstract:

Disclosed herein include systems, methods, compositions, and kits for measuring the secretion level of a secreted factor of a single cell. Disclosed herein include solid supports comprising a plurality of capture probes capable of specifically binding to secreted factors secreted by a single cell. In some of the embodiments, at least two of the capture probes are capable of binding different secreted factors. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe. Secreted factor-binding reagents can comprise a detectable moiety, or a precursor thereof. Secreted factor-binding reagents capable of binding the same secreted factor comprise the same detectable moiety, or a precursor thereof, and secreted factor-binding reagents capable of binding different secreted factors can comprise different detectable moieties, or precursors thereof.