Becton, Dickinson and Company
MAGNETIC CAPTURE BEAD MEDIATED MOLECULAR BARCODING OF NUCLEIC ACID TARGETS IN SINGLE PARTICLES AND COMPOSITIONS FOR USE IN THE SAME

Abstract:

Embodiments of the invention provide magnetic capture bead mediated methods of molecular barcoding nucleic acid targets of a particle, such as a cell or extracellular vesicle. Aspects of the methods include: a) combining a sample comprising the particle with a magnetic capture bead comprising a capture moiety for the particle to produce a captured sample; b) partitioning captured particles of the captured sample using an applied magnetic field mediated partitioning protocol to produce partitioned captured particles, wherein the partitioned captured particles are in spatial proximity to bead bound barcode nucleic acids comprising target binding regions; and c) lysing the partitioned captured particles so that nucleic acid acids released therefrom bind to the target binding regions to produce captured nucleic acids. Also provided are compositions, e.g., magnetic capture beads, including barcoded magnetic beads, as well as device/systems and kits, that find use in practicing embodiments of the methods.