Becton, Dickinson and Company
METHODS FOR DETERMINING PARTICLE SIZE AND LIGHT DETECTION SYSTEMS FOR SAME

Abstract:

Methods for determining a size of a particle in a flow stream from scattered light are described. Methods according to certain embodiments include detecting scattered light from a flow stream with two or more photodetectors, generating a data signal from the scattered light with each of the photodetectors, calculating a ratio of data signals from two or more of the photodetectors and determining the size of the particle based on the calculated ratio of the data signals. Light detection systems having two or more photodetectors for detecting scattered light from a flow stream are also provided. Integrated circuits (e.g., field programmable gate arrays) programmed to determine the size of a particle from scattered light data signals are also provided.