Becton, Dickinson and Company
LATERAL FLOW ASSAY AND METHODS FOR DETECTING HIGH CONCENTRATION ANALYTES

Abstract:

Sandwich-type lateral flow assay devices, kits, systems, and methods described herein include antibody-conjugated oversized particles that bind to analyte of interest in a sample and remain upstream of the capture zone when a fluid sample is applied to a lateral flow test device. Embodiments of the antibody-conjugated oversized particles allow for the precise determination of concentration of the analyte in the sample, including analyte present at high and very high concentrations. Lateral flow assays of the present disclosure can address drawbacks associated with the hook effect of sandwich-type lateral flow assays by eliminating the phase of the dose response curve where signal intensity is decreasing.