Becton, Dickinson and Company
DETERMINING EXPRESSIONS OF TRANSCRIPT VARIANTS AND POLYADENYLATION SITES

Abstract:

Disclosed herein include systems, methods, compositions, and kits for determining numbers of occurrences of variants (e.g., transcript variants) of targets (e.g., gene targets) in cells and/or samples. In some embodiments, modification target sites (e.g., polyadenylation sites) and usage thereof are determined. Whole transcriptome amplification analysis can be performed and the sequencing reads obtained can be analyzed to identify polyadenylation sites (and usage thereof) for the design of customized primer panels for targeted scRNAseq experiments.