Becton, Dickinson and Company
SINGLE CELL ASSAY FOR TRANSPOSASE-ACCESSIBLE CHROMATIN

Abstract:

Disclosed herein include systems, methods, compositions, and kits for labeling DNA (e.g., open chromatin-associated gDNA). The method can comprise contacting double-stranded DNA (dsDNA), such as gDNA, with a transposome to generate a plurality of dsDNA fragments each comprising a first 5' overhang and a second 5' overhang. The transposome can comprise a transposase, a first adaptor having a first 5' overhang, and a second adaptor having a second 5' overhang. The first 5' overhang can comprise a complement of a target-binding region of a bead oligonucleotide. The first 5' overhang can comprise a coupling sequence. The second 5' overhang can comprise a universal sequence. There are provided, in some embodiments, coupling oligonucleotides comprising a 5' complement of the coupling sequence and a 3' complement of a target-binding region of a bead oligonucleotide.