Becton, Dickinson and Company
Molecular label counting adjustment methods
Last updated:
Abstract:
Disclosed herein are methods and systems for determining the numbers of targets. In some embodiments, the method comprise: stochastically barcoding targets using stochastic barcodes; obtaining sequencing data; for one or more of the targets: counting the number of molecular labels with distinct sequences associated with the target in the sequencing data; identifying clusters of molecular labels of the target using directional adjacency; collapsing the sequencing data using the clusters of molecular labels of the target identified; and estimating the number of the target.
Status:
Grant
Type:
Utility
Filling date:
25 May 2017
Issue date:
26 Jul 2022