Bio-Rad Laboratories, Inc.
Droplet tagging contiguity preserved tagmented DNA

Abstract:

A method of maintaining contiguity in chromosomal DNA following treatment with a tagmentase. Conditions are selected such that the tagmentase does not release from the DNA, and thus forms a bridge linking DNA segments that have the same relationship (haplotype) as occurred in the genomic DNA. Thus the tagmentase step can occur in bulk (before partitions are formed). The resulting tagmentase-bridged DNA segments can be added to partitions maintaining the bridged segments until they are introduced into different partitions. Once in partitions, the contiguous DNA segments can be barcoded with a partition-specific barcode, thereby allowing for later identification of contiguous DNA after sequencing in bulk (after partitions contents are merged).