Bristol-Myers Squibb Company
METHODS OF PURIFYING PROTEINS USING CHROMATOGRAPHY

Abstract:

This disclosure provides a method of purifying proteins using a combined AEX-CEX chromatography in flow-through mode under an operating condition wherein no automation, engineering control, and/or in-line adjustment is used between the AEX and the CEX. The AEX and CEX (or CEX and AEX) can be connected directly.