Bristol-Myers Squibb Company
ONLINE BIOMASS CAPACITANCE MONITORING DURING LARGE SCALE PRODUCTION OF POLYPEPTIDES OF INTEREST

Abstract:

The present invention relates to the use of online biomass capacitance monitoring in perfusion cultures as a way to minimize volumes of cell culture media in large-scale manufacturing. In certain embodiments, a biomass capacitance probe is used to measure the viable cell density, and the viable cell density is used in conjunction with a pre-determined constant cell-specific perfusion rate to identify a target perfusion rate.