Bionano Genomics, Inc.
TARGET NUCLEIC ACID-DETECTING METHOD USING THREE-WAY JUNCTION STRUCTURE-INDUCED ISOTHERMAL AMPLIFICATION

Abstract:

The present invention relates to an isothermal nucleic acid amplification technique based on a three-way junction structure that is formed as a ThIsAmp template; a ThIsAmp primer; and a nucleic acid are associated with each other through a hybridization reaction. Beyond the limitation of the conventional EXPAR technique, that is, the restricted application range that the EXPAR can be used only for detecting short target nucleic acids having a 3'-OH end, the method according to the present invention can detect target nucleic acids at excellent efficiency without being limited to kinds of target nucleic acids.