Coherus BioSciences, Inc.
METHODS FOR PRODUCING RECOMBINANT PROTEINS

Abstract:

Provided herein are methods of producing a recombinant protein that include: providing a bacterium including a nucleic acid encoding a recombinant protein; and culturing the bacterium in a liquid culture medium including about 0.3 mM to about 300 mM Mg.sup.2+ under conditions sufficient for the production and release of the recombinant protein into the culture medium.