Editas Medicine, Inc.
SYNTHETIC GUIDE MOLECULES, COMPOSITIONS AND METHODS RELATING THERETO

Abstract:

Chemical syntheses of guide molecules are disclosed, along with compositions and methods relating thereto. A cost-effective and straightforward chemical synthesis of high-purity unimolecular guide molecules with minimal n-1 and/or n+1 species, truncation species, and other contaminants by providing, among other things, methods for synthesizing unimolecular guide molecules that involve cross-linking two or more pre-annealed guide fragments.