Guardant Health, Inc.
Methods for 3' overhang repair

Abstract:

Methods of repairing a partially double-stranded DNA fragment are provided. In some embodiments, the methods comprise (a) contacting the partially double-stranded DNA fragment with one or more primers of a primer population, wherein the partially double-stranded DNA fragment comprises a 3' overhang and the primer population comprises a random target-hybridizing sequence; (b) extending one or more primers of the primer population along the DNA fragment using a DNA polymerase, thereby producing one or more extended primers annealed to the DNA fragment; and (c) ligating the 3' end of one or more extended primers to the 5' end of an extended primer or a strand of the partially double-stranded DNA fragment, thereby providing a repaired DNA fragment.