Corning Incorporated
GRAVITY FLOW CELL CULTURE DEVICES, SYSTEMS, AND METHODS OF USE THEREOF

Abstract:

Multi-layer cell culture devices and systems for culturing suspension and adherent cells via continuous gravity flow of culture media without the use of motorized equipment minimize the risk of contamination of the cell culture. A multi-layer cell culture device includes at least first and second chambers configured to contain fluid and to be fluidly connected such that fluid in the first chamber unidirectionally and continuously flows by gravity flow from the first chamber and into the second chamber. A multi-layer cell culture device can further include a third chamber configured to contain fluid and positioned below the second chamber such that fluid in the second chamber unidirectionally and continuously flows by gravity flow from the second chamber and into the third chamber. Methods of culturing cells using these devices and systems are also described.