Illumina, Inc.
Systems and Devices for Characterization and Performance Analysis of Pixel-Based Sequencing

Abstract:

In one embodiment, a method of determining tag signals from measured intensities, the measured intensities collected by light sensors in a sensor array directed to a sample surface, the sample surface including pixel areas and holding a plurality of clusters during a sequence of sampling events, each light sensor directed to and measuring intensity from one of the pixel areas during each sampling period includes adjustments for background intensity and crosstalk and taking into account signal decay and phasing/pre-phasing. Coefficients for the adjustments can be determined by gradient descent, using as ground truth base calling by the system being characterized or by using reliable base calling of well-characterized sample run through the system being characterized.