Illumina, Inc.
SAMPLE ANALYZER FOR ANALYZING NUCLEIC ACID SEQUENCING DATA

Abstract:

Sample analyzer having a system controller that includes a plurality of modules. The plurality of modules include a first filter module that assigns sample reads to designated loci based on a sequence of nucleotides and an aligner module that analyzes the assigned reads to identify corresponding regions-of-interest (ROIs) within the assigned reads. A second filter module sorts the assigned reads based on the sequences of the ROIs such that the ROIs with different sequences are assigned as different potential alleles. Each potential allele has a sequence that is different from the sequences of other potential alleles within the designated locus. A stutter module analyzes the sequences of the potential alleles to determine whether a first allele of the potential alleles is suspected stutter product of a second allele of the potential alleles.