Illumina, Inc.
COMPOSITIONS AND METHODS FOR DETECTING AN ABASIC SITE

Abstract:

A method for detecting an abasic site is provided. The method may include flowing a solution over a substrate having a plurality of oligonucleotides coupled thereto. At least one of the oligonucleotides includes an abasic site. The solution may include a fluorophore coupled to a reactive group. The method may include reacting the reactive group with the abasic site to couple the fluorophore to the abasic site; and detecting the abasic site using fluorescence from the fluorophore.