Laboratory Corporation of America Holdings
Ion-Pairing Free LC-MS Bioanalysis of Oligonucleotides

Abstract:

Methods of LC-MS analysis of oligonucleotides free of ion-pairing reagents are disclosed herein. Historically, ion-pairing reagents have been used for acceptable extraction and chromatography prior to mass spectral analysis. The disclosure herein presents methods free of ion-pairing reagents at each stage, from extraction through the LC-MS endpoint. The methods improve assay reliability and reproducibility, extend column lifetimes, and decrease instrumental downtime previously necessary to remove ion-pairing reside. Also disclosed herein are systems for performing these methods.