Laboratory Corporation of America Holdings
Methods and Systems for Quantitative Detection of Antibodies

Abstract:

The present disclosure relates to methods, systems, kits, and computer-program products for the quantification of antibodies. In an embodiment of the disclosure, the method comprises generating a standard curve and subsequently utilizing the standard curve to quantitate an antibody of interest. Also disclosed are systems, kits, and computer-program products for the quantitative detection of an antibody of interest in a sample using the methods described herein. In certain embodiments, the standard curve is developed using an immunoglobulin (e.g., IgG) that does not specifically recognize the antigen(s) recognized by the antibody of interest but is of the same immunoglobulin class as the antibody of interest (IgG). In this way, an applicable standard curve may be generated for various isolates of the antibody of interest.