Pacific Biosciences of California, Inc.
COMPOSITIONS AND METHODS FOR IMPROVED cDNA SYNTHESIS

Abstract:

Modified template switching oligonucleotides (TSOs), compositions containing modified TSOs, and methods for employing modified TSOs to synthesize cDNA from RNA templates, where the cDNA includes an adapter region at the 3' end, are provided. The modified TSOs include at least one 2'-fluoro-ribonucleotide in the 3' annealing region and provide for improved conversion of RNA into full-length cDNA, resulting in increased yield and complexity as compared to non-modified TSOs and thereby finding use in generating cDNA from samples having low RNA input.