Pacific Biosciences of California, Inc.
REVERSIBLE MODIFICATION OF NUCLEOTIDES

Abstract:

Disclosed herein, inter alia, are methods for modifying a nucleotide, for example including reacting a nucleotide having a 3'-O-oxime moiety such as ##STR00001## with a reagent having an --ONH.sub.2 moiety to produce a nucleotide having a 3'-O--NH.sub.2 moiety such as ##STR00002## wherein the reagent having the --ONH.sub.2 moiety further comprises alkyl, alkenyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl, heteroalicyclyl, aralkyl, heteroaralkyl, or (heteroalicyclyl)alkyl.