Dr. Reddy's Laboratories Limited
Process for modifying galactosylation and G.sub.0F content of a glycoprotein composition by glutamine supplementation

Abstract:

The invention relates to a cell culture process for decreasing the galactosylated content and/or increasing the G0F content of a glycoprotein. The process involves subjecting recombinant cells expressing the said glycoprotein to a temperature and pH shift and supplementing cell culture with glutamine.