Regeneron Pharmaceuticals, Inc.
NON-HUMAN ANIMALS HAVING AN ENGINEERED IMMUNOGLOBULIN LAMBDA LIGHT CHAIN AND USES THEREOF

Abstract:

Non-human animals (and/or non-human cells) and methods of using the same are provided, which non-human animals (and/or non-human cells) have a genome comprising human antibody-encoding sequences (i.e., immunoglobulin genes). Non-human animals described herein express antibodies that contain immunoglobulin (Ig) light chains characterized by the presence of human V.lamda. domains. Non-human animals provided herein are, in some embodiments, characterized by expression of antibodies that contain human V.lamda. light chains that are encoded by human Ig.lamda. light chain-encoding sequences inserted into an endogenous Ig.kappa. light chain locus of said non-human animals Methods for producing antibodies from non-human animals are also provided, which antibodies contain human variable regions and mouse constant regions.