Regeneron Pharmaceuticals, Inc.
METHODS FOR BINDING SITE IDENTIFICATION USING HYDROGEN EXCHANGE MASS SPECTROMETRY

Abstract:

Methods for identifying a binding site between a protein pharmaceutical product and a host-cell protein (HCP) using hydrogen exchange mass spectrometry are provided. The present application also provides methods to modify protein pharmaceutical products to eliminate the cleavage or modification by HCPs. In addition, the present application provides methods to block the identified binding site in protein pharmaceutical products to eliminate the cleavage or modification by HCPs.