METHODS OF SIZE EXCLUSION CHROMATOGRAPHY FOR THE CHARACTERIZATION OF RECOMBINANT ADENO-ASSOCIATED VIRUS COMPOSITIONS

Abstract:

The present disclosure relates to using size exclusion chromatography to isolate AAV genome, to determine the vector genome size purity of a composition comprising isolated rAAV particles, to assess the folding or secondary structure of vector genomes inside the capsids, and to determine vector genome titer (Vg) of a composition comprising isolated rAAV particles.