Sanofi
METHODS FOR DETERMINING ADJUVANTED PROTEIN CONCENTRATION AND PERCENT ADSORPTION USING INTRINSIC FLUORESCENCE

Abstract:

Methods and systems for determining the concentration of compounds, such as proteins, in compositions comprising adjuvanted complexes of the compounds are provided. The methods and systems generally comprise (i) measuring the intrinsic fluorescence of a composition comprising adjuvanted complexes of the compound and (ii) comparing the measured fluorescence intensity value to a calibration curve prepared using known concentrations of the compound. Also provided are methods and systems for determining percent adsorption of a selected compound to an adjuvant.