SQZ Biotechnologies Company
MICROFLUIDIC CHIP HAVING INCREASED THROUGHPUT FOR USE IN A SYSTEM FOR DELIVERY OF A PAYLOAD INTO A CELL

Abstract:

Provided is a microfluidic chip for causing the delivery of a payload to a cell comprising a first layer, a second layer, and a fluid flow region between the first layer and the second layer. This microfluidic chip is configured to accept flow of a cell suspension into the fluid flow region. The first layer of the microfluidic chip comprises a protrusion extending toward the second layer to form a constriction between the protrusion and the second layer, wherein the constriction is configured to cause perturbation of a cell membrane of a cell of the cell suspension as the cell passes through the constriction.