Savara Inc.
SEPARATION OF VANCOMYCIN AND ITS DEGRADATION PRODUCTS

Abstract:

Disclosed is a chromatographic method for separating a mixture of compounds having ionizable groups using a mobile phase comprising (a) a first mobile phase component comprising an aqueous buffer system and an organic solvent mixture miscible with water, and (b) a second mobile phase component comprising an aqueous buffer system and an organic solvent mixture miscible with water, wherein the buffer system and the solvent mixture in the first mobile phase component are different from the buffer system and the solvent mixture in the second mobile phase component and the ratio of the first mobile phase component to the second mobile phase component is varied during the separation. The method can be used for the separation of vancomycin and its degradation products.