Takeda Pharmaceutical Company Limited
METHOD FOR CULTURE OF CELLS

Abstract:

As a technique for proliferating neural crest cells without reducing differentiation capacity, provided is a method for producing neural crest cells, comprising the steps of: (1) obtaining neural crest cells; and (2) suspension-culturing the neural crest cells in a medium comprising a GSK3.beta. inhibitor and a basic fibroblast growth factor (bFGF), wherein the medium comprises the GSK3.beta. inhibitor with a concentration that exhibits an effect equivalent to that exhibited by CHIR99021 with a concentration of higher than 1 .mu.M and lower than 5 .mu.M.