Takeda Pharmaceutical Company Limited
Skeletal Muscle Precursor Cells and Production Method for Skeletal Muscle Cells

Abstract:

The present invention relates to a method for producing skeletal muscle cells from pluripotent stem cells. More specifically, the present invention relates to: a method comprising culturing pluripotent stem cells in a medium containing a GSK3.beta. inhibitor, a medium containing an ALK inhibitor, and a medium containing a cAMP inducer to induce differentiation into skeletal muscle precursor cells; a method comprising culturing the obtained skeletal muscle precursor cells in a medium containing a growth factor and/or a medium free from a growth factor to induce differentiation into skeletal muscle cells; and a frozen cell preparation comprising the obtained skeletal muscle precursor cells.