Takeda Pharmaceutical Company Limited
QUANTITATIVE PCR PROBE

Abstract:

To provide a qPCR probe and the like as a novel means for enabling the detection of human cells transplanted or administered to non-human animals. A quantitative PCR probe comprising: a sequence hybridizable with an oligonucleotide consisting of a sequence of 10 to 35 contiguous bases from within a predetermined nucleotide sequence derived from the human LINE-1 gene, and a labeling part. The quantitative PCR probe can be used, for example, in the production of a quantitative PCR kit further comprising a forward primer derived from human LINE-1 and a reverse primer derived from human LINE-1 (the individual sequences are in a predetermined positional relationship).