Takeda Pharmaceutical Company Limited
QUANTITATIVE FLOW CYTOMETRY

Abstract:

The present disclosure relates to a method for measuring the cell concentration in a sample, comprising the following steps: (1) measuring the concentrations of cells in standard samples each having a known concentration using a flow cytometer; (2) preparing a calibration curve based on the values measured in step (1) and the known concentrations; (3) measuring the concentration of cells in a sample having an unknown concentration using a flow cytometer; and (4) determining the concentration of cells from the value measured in step (3) based on the calibration curve prepared in step (2).