Takeda Pharmaceutical Company Limited
COMPOSITION FOR MODIFYING TARGET GENE

Abstract:

The present invention provides a delivery technique for delivering a gene modification tool capable of providing a high gene modification efficiency in cells. The composition according to the present invention is a composition for inducing gene modification at a target gene locus in a cell, the composition containing 1) a compound represented by formula (I) or a salt thereof; 2) a structural lipid; and 3) a guide RNA or a DNA including a sequence encoding the guide RNA, and/or an RNA-guided nuclease or a nucleic acid including a sequence encoding the RNA-guided nuclease. In formula (I), n represents an integer of 2 to 5, R represents a linear C.sub.1-5 alkyl group, a linear C.sub.7-11 alkenyl group, or a linear C.sub.11 alkadienyl group, and wavy lines each independently represent a cis-type bond or a trans-type bond. ##STR00001##