Takeda Pharmaceutical Company Limited
METHODS OF PRODUCING AN ANTI-a4B7 ANTIBODY

Abstract:

Provided herein are methods for purifying an anti-.alpha.4.beta.7 integrin antibody, such as vedolizumab, from a liquid solution, e.g., from a mammalian cell culture clarified harvest. The invention relates, inter alia, to purification methods for controlling the amount of product-related substances and/or process-related impurities present in purified preparations of an anti-.alpha.4.beta.7 integrin antibody, or antigen-binding fragment thereof, e.g., vedolizumab. Compositions comprising an anti-.alpha.4.beta.7 antibody, and uses thereof to treat a disorder, are also provided.