10x Genomics, Inc.
NUCLEIC ACID ENRICHMENT WITHIN PARTITIONS

Abstract:

The present disclosure provides methods and systems for sample processing or analysis. A method for processing a sample may comprise co-partitioning an enzyme and a biological particle comprising a first set of molecules (e.g., RNA molecules comprising one or more mRNA molecules) and a second set of molecules (e.g., RNA molecules other than mRNA molecules) in a partition, lysing or permeabilizing the biological particle to provide access to the first set of molecules and the second set of molecules, and selectively digesting molecules of the second set of molecules with the enzyme to increase a concentration or amount of the first set of molecules relative to the second set of molecules within the partition.