10x Genomics, Inc.
SYSTEMS AND METHODS FOR USING THE SPATIAL DISTRIBUTION OF HAPLOTYPES TO DETERMINE A BIOLOGICAL CONDITION

Abstract:

A method determining a biological condition of a subject using a spatial distribution of haplotypes is provided in which sequence reads are obtained from a two-dimensional array of positions on a substrate upon contacting a biological sample of the subject with the two-dimensional array of positions on the substrate. Each capture probe plurality in a set of capture probe pluralities is at a different position in the two-dimensional array, associates with one or more analytes from the biological sample, and has a corresponding spatial barcode from a plurality of spatial barcodes. Each sequence read includes a spatial barcode of the corresponding capture probe plurality. The barcoded sequence reads are used to quantify each haplotype for each of a plurality of loci thereby determining the spatial distribution of the one or more haplotypes in the biological sample which, in turn, is used to characterize the biological condition of the subject.