10x Genomics, Inc.
METHOD FOR ISOLATING NUCLEI AND CELLS FROM TISSUES

Abstract:

A method of extracting and isolating fixed biological particles, such as fixed cells and/or fixed nuclei from snap-frozen biological tissue yields dramatically improved amounts of ligation products and an increase in targeted single cell RNA sequence data quality and sensitivity in subsequent RNA-templated ligation. The method preserves the integrity of the biological particles, such as cells and/or or nuclei from biological tissue samples during extraction and isolation by reducing the amount of cell degradation and RNA leakage associated with conventional methods.