10x Genomics, Inc.
METHODS AND SYSTEMS FOR BARCODING MULTIPLE NUCLEIC ACID ANALYTES

Abstract:

Methods, kit, systems, and compositions for processing nucleic acids and barcoding nucleic acids are disclosed. The methods and systems generally may comprise the presence of a support comprising nucleic acid barcode molecules which may be used to interact with nucleic acids to generate barcoded nucleic acid molecules. The support may have a plurality of nucleic acid barcode molecules that are able to barcode multiple analytes. This may allow the identification of multiple types of analytes and correlating the analytes as originating from a same biological particle. A splint and primer nucleic acids may also be used to generate barcoded nucleic acids. The methods and systems can be applied to a variety of biological samples and can analyze different nucleic acids, proteins, or other macromolecules of the biological samples.